Female TRT Member Guide

Everything you need to get started & successfully manage your HRT program

Testosterone Injection & Preparation

 Review this section before your first injection.

Supplies Needed
  • 1mL Luer lock syringe
  • Vial topper or 21g draw needles
  • 25G 1-inch needle (IM)
  • 27G 1/2-inch needle (SubQ or shallow deltoid)
  • Alcohol pads
  1. Wash hands.
  2. Wipe vial top with alcohol.
  3. Push vial topper into place. (only once per vial)
  4. Draw air into syringe (match dose volume + 3 units).
  5. Inject air into vial.
  6. Draw medication.
  7. Remove air bubbles.
  8. Adjust to prescribed dose.

In order of recommended location but do what you are most comfortagle with:

  • Ventrogluteal (preferred IM)
  • Dorso Glute (buttock)
  • Deltoid
  • Subcutaneous abdomen
  • thigh (quad)
 
Rotate sides weekly. For example if you are doing vent glute on the right side one week, then rotate to the left hip the following.

What to Expect (First 12 Weeks)

TRT is a physiologic optimization process. Improvements occur gradually over several weeks to months.

 

Weeks 1–2

Subtle energy shifts
Mild water retention
Possible mood variability

Improved libido
Better drive and motivation
Early strength improvements

More consistent energy
Body composition changes
Performance gains

Mild acne
Slight fluid retention

Severe swelling
Injection site infection
Significant mood instability

Lab Timing & Monitoring

Monitoring is mandatory to continue therapy

First Followup Monitoring Labs

First Monitoring Labs 

  • Injections: 8 weeks after starting injections
  • Creams: 6 weeks for testosterone cream
  • Pellets: 4 weeks after procedure

 

Labs are drawn:

  • Injections: The day before injection OR the morning of injection before administering your injection
  • Creams: The day of labs you will not apply your testosterone cream in the morning 
  1. Total testosterone
  2. Free testosterone
  3. SHBG
  4. DHEA (if prescribed)

Follow-up labs are mandatory. Failure to complete labs as scheduled will result in prescription being withheld.

For injectable testosterone, levels rise after your injection and gradually decline until your next dose.

We measure labs at the trough, which is the lowest point before your next injection.

This allows us to compare results consistently over time — apples to apples.

If labs are drawn immediately after an injection, levels will appear artificially elevated. Adjusting your dose based on a peak value can lead to inaccurate changes in your treatment plan.

For consistency and safe optimization, labs should always be drawn:

  • The day before your scheduled injection, or
  • The morning of your injection before administering your dose

 

Maintaining consistent timing ensures accurate comparisons and appropriate dose adjustments.

Refills

Medication volume and dose determine refill timing.

How Long Does a Vial Last?

Most 10mL testosterone vials at 60 units (120mg) per week lasts approximately 14 weeks. If your dose is 200mg / week, this will last you 10 weeks or 70 days. Your prescription will say the “days supply”. 

Please set a reminder based on this calculation for when you will need to request a refill. WE DO NOT automatically send refills. This must be requested. 

  1. The easiest way is to request in the patient portal HERE. If your dose has changed you can request in the portal, but make a note and confirm if there have been any address changes as well.
  2. Notify clinic at least 5 business days before running out.
  3. Labs must be current.
  4. Follow-ups must be current.

Follow-up labs are mandatory. Failure to complete labs as scheduled will result in prescription being withheld.

Required Follow-Ups

Required Visits

Followup visits

Patients must be seen in clinic at least once every 6 months to receive refills.

This is mandatory for continued prescribing.

When starting your treatment you will be seen more often. Often at a 6-8 week interval. Once you are feeling good followups can be less often, we still recommend visits every three months to be proactie about your care.

Membership Benefits

The following are Included in your membership
  • Testosterone medication
  • Injection supplies (syringes, needles, alcohol swabs, vial toppers)
  • Monitoring labs
  • One annual comprehensive panel
  • One wellness injection per month
  • Provider follow-ups
  • Preferred pricing on peptides
  • Recovery suite access (coming soon)

 

Other Member Perks:

  • Discounted peptides
  • Add-on optimization labs

Clinic Address

Spark Hormone Therapy 6500 29th Street, Suite 280 Greeley, CO 80634

Phone

Click to Call: (970) 714-3572

Directions

Use the interactive map below for navigation to our Greeley clinic.

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